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多克隆抗體定制服務(wù)

多克隆抗體存在于免疫動(dòng)物的血清中,可通過(guò)直接分離血清獲得。由于具有可識別多個(gè)表位、可引起凝集反應、沉淀反應等優(yōu)點(diǎn),被廣泛應用于免疫學(xué)診斷領(lǐng)域。比如經(jīng)常用到的Western blotting實(shí)驗中的酶標二抗和ELISA實(shí)驗中的包被抗體。而且制備費用也較單克隆抗體低。

華美生物自主建立抗體研發(fā)生產(chǎn)平臺,可為您提供可靠的多克隆抗體定制服務(wù),并有多種免疫原和多種宿主物種可供選擇。您可以選擇自己提供抗原或者我們幫您設計并制備抗原,我們將提供給您滿(mǎn)足您實(shí)驗需求的多克隆抗體產(chǎn)品。

服務(wù)流程:

多克隆抗體定制服務(wù)流程

服務(wù)內容及周期:

免疫原 服務(wù)內容 交付內容 服務(wù)周期
多肽
  • 抗原制備
  • 動(dòng)物免疫
  • 血清滴度檢測
  • 親和純化或Protein A/G純化
  • 抗原 WB 驗證
  1. 提供抗原QC報告;
  2. ELISA滴度效價(jià)保證1:64000;
  3. 抗原WB檢測結果保證陽(yáng)性;
  4. 提供1mL免疫前血清,2mL抗血清,5-10mg經(jīng)protein A/G或親和純化的抗體;
  5. 提供SDS-PAGE檢測結果,抗體純度保證90%。
12-14 周
重組蛋白 14-16 周
天然蛋白 12-14 周

服務(wù)實(shí)例:

ELISA
Antigen coating concentration 2 ug/ml
Antiserum 1:2000 is more than diluted
Secondary:Goat polyclonal to rabbit IgG at 1/50000 dilution

Western Blot
Positive WB detected in:K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate,Rat liver tissue, Mouse brain tissue, Mouse lung tissue
All lanes:a antibody at 2ug/ ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa

Western Blot
WT: Wild-type 293 cells
KO: Knockout 293 cells

IHC image of A antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with A antibody at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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