Your Good Partner in Biology Research

EGFR

EGFR(Epidermal Growth Factor Receptor)是一個(gè)經(jīng)典的跨膜蛋白,屬于酪氨酸激酶型受體。EGFR廣泛分布于哺乳動(dòng)物上皮細胞、成纖維細胞、膠質(zhì)細胞、角質(zhì)細胞等細胞表面,EGFR信號通路對細胞的生長(cháng)、增殖和分化等生理過(guò)程發(fā)揮重要的作用。EGFR通過(guò)與配體結合而激活,介導上皮生長(cháng)因子(EGF)細胞增殖和信號傳導。EGFR被配體激活后啟動(dòng)胞內信號傳導,經(jīng)過(guò)細胞質(zhì)中銜接蛋白、酶的級聯(lián)反應,轉錄因子激活基因的轉錄,介導細胞遷移和黏附等過(guò)程,且與腫瘤的形成和惡化密切相關(guān)。許多研究表明EGFR在非小細胞肺癌、頭頸部腫瘤、乳腺癌、膀胱癌、結直腸癌、卵巢癌等多種腫瘤中存在突變或高表達。因此,已經(jīng)有很多研究把EGFR作為腫瘤的治療靶點(diǎn)。近年來(lái),靶向EGFR藥物已成為抗腫瘤藥物研究的熱點(diǎn)。

熱銷(xiāo)產(chǎn)品

Recombinant Human Epidermal growth factor receptor(EGFR),partial (Active) CSB-MP007479HU

驗證數據

High Purity Validated by
SDS-PAGE

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC50 of human EGFR protein is 0.6919-1.047 ng/ml.

Excellent Bioactivity Validated by LSPR Assay

Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.

EGFR Recombinant Monoclonal Antibody CSB-RA159341MA1HU

驗證數據

IHC
CSB-RA159341MA1HU IHC

IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

FC
CSB-RA159341MA1HU FC

Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

EGFR Antibodies

EGFR for Homo sapiens (Human)

EGFR for H

EGFR Proteins

EGFR Proteins for Gallus gallus (Chicken)

EGFR Proteins for Apis mellifera (Honeybee)

EGFR Proteins for Macaca mulatta (Rhesus macaque)

EGFR Proteins for Drosophila melanogaster (Fruit fly)

EGFR Proteins for Homo sapiens (Human)

EGFR ELISA Kit

EGFR ELISA Kit for Homo sapiens (Human)