CD274

PD-1——重要的免疫檢查點(diǎn)

CD274（PDCD1配體1，又稱PD-L1或B7-H1）是重要的免疫檢查點(diǎn)蛋白。PD-L1作為PD-1的配體之一，PD-L1能夠與PD-1結(jié)合，抑制T細(xì)胞增殖以及細(xì)胞因子分泌，負(fù)調(diào)控淋巴細(xì)胞的激活。PD-L1除了會(huì)表達(dá)在腫瘤表面，參與免疫逃逸，還會(huì)在IFN-γ刺激下，表達(dá)在抗原提呈細(xì)胞（DC細(xì)胞、巨噬細(xì)胞等），以及血管內(nèi)皮細(xì)胞的表面。大量研究發(fā)現(xiàn)PD-1和PD-L1信號(hào)通路在感染免疫、腫瘤免疫、移植免疫中起著重要作用。尤其在腫瘤中，PD-L1與PD-1的結(jié)合，可誘導(dǎo)T細(xì)胞凋亡、失能、耗竭，進(jìn)而抑制腫瘤抗原特異性CD8+T細(xì)胞的激活、增殖和抗腫瘤功能，實(shí)現(xiàn)腫瘤免疫逃逸，然而阻斷PD-1／PD-L1通路可以恢復(fù)免疫系統(tǒng)對(duì)腫瘤細(xì)胞的殺傷作用。因此，靶向PD-1/PD-L1信號(hào)通路成為治療腫瘤的研究熱點(diǎn)。

Recombinant Human CD274 活性蛋白實(shí)驗(yàn)驗(yàn)證數(shù)據(jù)

High Purity Validated by SDS-PAGE

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC₅₀ of human PD-L1 protein is 1.252-1.653 ng/mL.