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Recombinant Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active)

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  • 中文名稱：
    人GPRC5D重組蛋白
  • 貨號：
    CSB-MP882153HU
  • 規(guī)格：
  • 圖片：
    • CSB-MP882153HU is detected by Mouse anti-6*His monoclonal antibody.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC50 is 8.847-10.38 ng/mL. Biological Activity Assay
  • 其他：

產品詳情

  • 內毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC50 is 8.847-10.38 ng/mL.
  • 基因名：
  • Uniprot No.：
  • 分子結構：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    Mammalian cell
  • 分子量：
    40.6 kDa
  • 表達區(qū)域：
    1-345aa
  • 氨基酸序列
    MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV
  • 蛋白標簽：
    C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
  • 產品提供形式：
    Lyophilized powder
    Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述：

    This recombinant human GPRC5D protein is generated by the HEK293 cells expression systemand is expressed with sequence Met1-Val345 of human GPRC5D fused with a 10xHis-tag at the C-terminal. It is a virus-like particle. Its endotoxin and biological activity have been measured.

    GPRC5D is highly expressed on the surface of primary multiple myeloma (MM) cells, while its expression in normal tissues is limited to the hair follicle. GPRC5D thus becomes an attractive target for the treatment of MM and is expected to provide new therapies for MM patients with poor prognoses. The treatment strategy of GPRC5D in MM mainly focuses on GPRC5D-CAR T cell therapy and GPRC5D-combined with bispecific antibody therapy. Currently, there are several combination drugs targeting GPRC5D or based on GPRC5D in clinical development. For example, Talquetamab (JNJ-64407564) is a GPRC5D/CD3 bispecific antibody that inhibits tumor growth by recruiting and activating CD3-positive T cells and inducing T cells to kill GPRC5D-positive MM cells.

  • Datasheet & COA：
    Please contact us to get it.

產品評價

平均分:
5.0分 - 1 個評價

實驗應用：結合實驗/蛋白間互作

產品評價： 我使用CSB-MP882153HU產品進行抗體篩選，篩選的陽性細胞株特異性好，靈敏度高，空白對照背景低，實驗很順利，極大地節(jié)省了時間。

By 鐘老師

靶點詳情

  • 基因功能參考文獻：
    1. GPRC5D in MM cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. PMID: 23510526
    2. Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma. PMID: 22591013
  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 3 family
  • 組織特異性：
    Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscl
  • 數據庫鏈接：

    HGNC: 13310

    OMIM: 607437

    KEGG: hsa:55507

    STRING: 9606.ENSP00000228887

    UniGene: Hs.644599